Review



polyvinylidene difluoride membranes  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Bio-Rad polyvinylidene difluoride membranes
    Polyvinylidene Difluoride Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 2910 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/Trans-Blot+Turbo+Mini+PVDF+Transfer+Packs/pmc13018931-112-23-27
    Average 96 stars, based on 2910 article reviews
    polyvinylidene difluoride membranes - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Conformational dynamics of SARS-CoV-2 spike on a membrane reveals the allosteric effects of furin cleavage and the D614G mutation.
    Article Snippet: Samples were then heated at 98°C for 20 min. After spinning, 10 μl of the boiled sample was added to a 4 to 20% Mini- PROTEAN TGX gel (Bio- Rad, 4561095) and run at 200 V for 45 min in tris/glycine/ SDS buffer (Bio- Rad, 1610732). .. The proteins in the gel were transferred to a 0.2- μm polyvinylidene difluoride membrane (Bio- Rad, 1620174) by first briefly rinsing the membrane in methanol and then water followed by a 10- min incubation in Towbin’s buffer containing 20% methanol. ..

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Protein samples were then run on a 4%–12% NuPAGE Bis-Tris gel (#NP0336BOX, Thermo Fisher) at 175 V for 45 min. .. The gel was then transferred onto a polyvinylidene difluoride membrane (#1620174, Bio-Rad) and blocked with 5% milk in TBST for ≥1 h. The membrane was incubated in a dilution of 1:1,000 anti-mCherry monoclonal antibody (#M11217, Thermo Fisher) or 1:1,000 anti-GFP polyclonal antibody (#632592, Takara Bio) in blocking solution for ≥1 h, rinsed five times with TBST, and then incubated in 1:10,000 diluted anti-rat or anti-rabbit secondary antibodies labeled with IRDye 680RD or 800CW (#926-68076 and #925-32211, LI-COR Biosciences). .. The membrane was rinsed three times in TBST, once in TBS, and imaged on a LI-COR Odyssey CLx imager (LI-COR Biosciences).

    Article Title: Switching off CK2-mediated activation of survivin offers new therapeutic opportunities in neuroblastoma.
    Article Snippet: .. The proteins were then blotted on a polyvinylidene difluoride membrane, which was blocked in 5% skimmed milk and 0.1% Tween 20 in TBS for 2 h at room temperature and then treated with the appropriate primary (4 °C overnight) and secondary (2 h at room temperature) antibodies and revealed by chemiluminescence (ECL Bio-Rad). ..

    Article Title: Systemic delivery of AAV-GFM1 corrects COXPD1 molecular alterations in Gfm1 R671C/- mice.
    Article Snippet: .. In both cases, the protein extracts (20–30 μg) were denatured at 95 °C for 5 min, resolved on 10–12% polyacrylamide gel at 120 V for 120 min and blotted to a polyvinylidene difluoride membrane (Immun-Blot® PVDF membrane, Bio-Rad) at 400 mA for 1 h at 4 °C. ..

    Article Title: Compositions and methods for increasing cancer cell sensitivity to alternating electric fields
    Article Snippet: Samples were run on SDS-polyacrylamide gel electrophoresis (Bolt 8% Bis-Tris base gel NW00080BOX, Thermo-Fischer). .. After electrophoresis, proteins were transferred to 0.2 μm polyvinylidene difluoride membrane (Immuno-Blot PVDF #162-0177, Bio-Rad) and probed with the appropriate primary antibody: GAPDH (SC-32233, Santa Cruz), Vinculin (ab140007, Abcam), Cyclin D1 (2922, Cell Signaling), Rb (9309, Cell Signaling), pRb S807/811 (8516, Cell Signaling), pAkt 5473, (4051, Cell signaling), Akt (2920, Cell signaling)), FAK (3283S, Cell signaling), Phospho-FAK (Tyr397) (3283S, Cell signaling, Src (2109S, cell signaling) and Phospho-Src (Tyr416) (2101S, Cell signaling). followed by horseradish peroxidase-conjugated secondary antibody (goat anti rabbit 7074, Cell Signaling and goat anti mouse 7076, Cell Signaling) and a chemiluminescent substrate (WBLUF0100, Signa-Aldrich). ..

    Article Title: Conformational dynamics of SARS-CoV-2 spike on a membrane reveals the allosteric effects of furin cleavage and the D614G mutation
    Article Snippet: Samples were then heated at 98°C for 20 min. After spinning, 10 μl of the boiled sample was added to a 4 to 20% Mini-PROTEAN TGX gel (Bio-Rad, 4561095) and run at 200 V for 45 min in tris/glycine/SDS buffer (Bio-Rad, 1610732). .. The proteins in the gel were transferred to a 0.2-μm polyvinylidene difluoride membrane (Bio-Rad, 1620174) by first briefly rinsing the membrane in methanol and then water followed by a 10-min incubation in Towbin’s buffer containing 20% methanol. ..

    Article Title: Hypokalaemia and bradycardia unmask the loss-of-function phenotype of a Brugada Syndrome SCN5A mutation
    Article Snippet: The protein extracts were then heated at 99°C for 5 min in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) solubilising buffer (58 mM Tris HCl, 10% glycerol, 2% SDS, 0.004% bromophenol blue, pH 6.8) containing 2.5% β-mercaptoethanol (Life-Technologies, #31350010). .. The proteins (5 μg/lane) were separated by means of SDS-PAGE on a 4–12% polyacrylamide gel (NuPAGETM Bis-Tris, Life Technologies, #NW04120 Box) and transferred to a polyvinylidene difluoride membrane (PVDF, Bio-Rad, # 1620177). .. Blotted proteins were exposed to tris-buffered saline with Tween 20 (TBST) blocking solution (mM): Tris HCl 20, NaCl 150, pH 7.5, Tween20 0.1% plus 5% milk powder (Blotting Grade Blocker Non-Fat Dry Milk; Bio-Rad, #170-6404), and the membrane was then incubated overnight at 4°C with the anti-Na v 1.5 (Sigma-Aldrich; dilution 1:250, #S1946) or the anti-actin primary antibodies (Sigma Aldrich, dilution 1:3000, #A3853) diluted in the blocking buffer.

    Article Title: Matrix Gla Protein Expression in Pericytes and Myofibroblasts Contributes to Renal Fibrosis
    Article Snippet: .. Serum immunoprecipitants or kidney lysates were resolved on Tris-glycine SDS-PAGE gel, transferred onto a 0.2-μm polyvinylidene difluoride membrane (1620177; Bio-Rad, Hercules, CA) and were detected using an anti-HA antibody (1:1000; 3724; Cell Signaling Technologies), anti–T cell immunoglobulin cumin 1/kidney injury molecule-1/hepatitis A virus cellular receptor (TIM-1/KIM-1/HAVCR) antibody (1:1000; AF1817; Novus Biologicals, Littleton, CO), or anti–β-tubulin antibody (1:1000; 2146; Cell Signaling Technologies) as primary antibodies and rabbit anti–goat–horseradish peroxidase IgG (1:1000; HAF017; Novus Biologicals) or anti-rabbit IgG horseradish peroxidase–linked antibody (1:2000; 7074; Cell Signaling Technologies) as the secondary antibody. .. Blood urea nitrogen and serum creatinine were measured using the QuantiChrom Urea Assay Kit (DIUR-100; BioAssay Systems, Hayward, CA) and Creatinine Assay Kit (265-30; Sekisui Diagnostics P.E.I.

    Incubation:

    Article Title: Conformational dynamics of SARS-CoV-2 spike on a membrane reveals the allosteric effects of furin cleavage and the D614G mutation.
    Article Snippet: Samples were then heated at 98°C for 20 min. After spinning, 10 μl of the boiled sample was added to a 4 to 20% Mini- PROTEAN TGX gel (Bio- Rad, 4561095) and run at 200 V for 45 min in tris/glycine/ SDS buffer (Bio- Rad, 1610732). .. The proteins in the gel were transferred to a 0.2- μm polyvinylidene difluoride membrane (Bio- Rad, 1620174) by first briefly rinsing the membrane in methanol and then water followed by a 10- min incubation in Towbin’s buffer containing 20% methanol. ..

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Protein samples were then run on a 4%–12% NuPAGE Bis-Tris gel (#NP0336BOX, Thermo Fisher) at 175 V for 45 min. .. The gel was then transferred onto a polyvinylidene difluoride membrane (#1620174, Bio-Rad) and blocked with 5% milk in TBST for ≥1 h. The membrane was incubated in a dilution of 1:1,000 anti-mCherry monoclonal antibody (#M11217, Thermo Fisher) or 1:1,000 anti-GFP polyclonal antibody (#632592, Takara Bio) in blocking solution for ≥1 h, rinsed five times with TBST, and then incubated in 1:10,000 diluted anti-rat or anti-rabbit secondary antibodies labeled with IRDye 680RD or 800CW (#926-68076 and #925-32211, LI-COR Biosciences). .. The membrane was rinsed three times in TBST, once in TBS, and imaged on a LI-COR Odyssey CLx imager (LI-COR Biosciences).

    Article Title: Conformational dynamics of SARS-CoV-2 spike on a membrane reveals the allosteric effects of furin cleavage and the D614G mutation
    Article Snippet: Samples were then heated at 98°C for 20 min. After spinning, 10 μl of the boiled sample was added to a 4 to 20% Mini-PROTEAN TGX gel (Bio-Rad, 4561095) and run at 200 V for 45 min in tris/glycine/SDS buffer (Bio-Rad, 1610732). .. The proteins in the gel were transferred to a 0.2-μm polyvinylidene difluoride membrane (Bio-Rad, 1620174) by first briefly rinsing the membrane in methanol and then water followed by a 10-min incubation in Towbin’s buffer containing 20% methanol. ..

    Blocking Assay:

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Protein samples were then run on a 4%–12% NuPAGE Bis-Tris gel (#NP0336BOX, Thermo Fisher) at 175 V for 45 min. .. The gel was then transferred onto a polyvinylidene difluoride membrane (#1620174, Bio-Rad) and blocked with 5% milk in TBST for ≥1 h. The membrane was incubated in a dilution of 1:1,000 anti-mCherry monoclonal antibody (#M11217, Thermo Fisher) or 1:1,000 anti-GFP polyclonal antibody (#632592, Takara Bio) in blocking solution for ≥1 h, rinsed five times with TBST, and then incubated in 1:10,000 diluted anti-rat or anti-rabbit secondary antibodies labeled with IRDye 680RD or 800CW (#926-68076 and #925-32211, LI-COR Biosciences). .. The membrane was rinsed three times in TBST, once in TBS, and imaged on a LI-COR Odyssey CLx imager (LI-COR Biosciences).

    Labeling:

    Article Title: Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.
    Article Snippet: Protein samples were then run on a 4%–12% NuPAGE Bis-Tris gel (#NP0336BOX, Thermo Fisher) at 175 V for 45 min. .. The gel was then transferred onto a polyvinylidene difluoride membrane (#1620174, Bio-Rad) and blocked with 5% milk in TBST for ≥1 h. The membrane was incubated in a dilution of 1:1,000 anti-mCherry monoclonal antibody (#M11217, Thermo Fisher) or 1:1,000 anti-GFP polyclonal antibody (#632592, Takara Bio) in blocking solution for ≥1 h, rinsed five times with TBST, and then incubated in 1:10,000 diluted anti-rat or anti-rabbit secondary antibodies labeled with IRDye 680RD or 800CW (#926-68076 and #925-32211, LI-COR Biosciences). .. The membrane was rinsed three times in TBST, once in TBS, and imaged on a LI-COR Odyssey CLx imager (LI-COR Biosciences).

    Electrophoresis:

    Article Title: Compositions and methods for increasing cancer cell sensitivity to alternating electric fields
    Article Snippet: Samples were run on SDS-polyacrylamide gel electrophoresis (Bolt 8% Bis-Tris base gel NW00080BOX, Thermo-Fischer). .. After electrophoresis, proteins were transferred to 0.2 μm polyvinylidene difluoride membrane (Immuno-Blot PVDF #162-0177, Bio-Rad) and probed with the appropriate primary antibody: GAPDH (SC-32233, Santa Cruz), Vinculin (ab140007, Abcam), Cyclin D1 (2922, Cell Signaling), Rb (9309, Cell Signaling), pRb S807/811 (8516, Cell Signaling), pAkt 5473, (4051, Cell signaling), Akt (2920, Cell signaling)), FAK (3283S, Cell signaling), Phospho-FAK (Tyr397) (3283S, Cell signaling, Src (2109S, cell signaling) and Phospho-Src (Tyr416) (2101S, Cell signaling). followed by horseradish peroxidase-conjugated secondary antibody (goat anti rabbit 7074, Cell Signaling and goat anti mouse 7076, Cell Signaling) and a chemiluminescent substrate (WBLUF0100, Signa-Aldrich). ..

    SDS Page:

    Article Title: Hypokalaemia and bradycardia unmask the loss-of-function phenotype of a Brugada Syndrome SCN5A mutation
    Article Snippet: The protein extracts were then heated at 99°C for 5 min in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) solubilising buffer (58 mM Tris HCl, 10% glycerol, 2% SDS, 0.004% bromophenol blue, pH 6.8) containing 2.5% β-mercaptoethanol (Life-Technologies, #31350010). .. The proteins (5 μg/lane) were separated by means of SDS-PAGE on a 4–12% polyacrylamide gel (NuPAGETM Bis-Tris, Life Technologies, #NW04120 Box) and transferred to a polyvinylidene difluoride membrane (PVDF, Bio-Rad, # 1620177). .. Blotted proteins were exposed to tris-buffered saline with Tween 20 (TBST) blocking solution (mM): Tris HCl 20, NaCl 150, pH 7.5, Tween20 0.1% plus 5% milk powder (Blotting Grade Blocker Non-Fat Dry Milk; Bio-Rad, #170-6404), and the membrane was then incubated overnight at 4°C with the anti-Na v 1.5 (Sigma-Aldrich; dilution 1:250, #S1946) or the anti-actin primary antibodies (Sigma Aldrich, dilution 1:3000, #A3853) diluted in the blocking buffer.

    Article Title: Matrix Gla Protein Expression in Pericytes and Myofibroblasts Contributes to Renal Fibrosis
    Article Snippet: .. Serum immunoprecipitants or kidney lysates were resolved on Tris-glycine SDS-PAGE gel, transferred onto a 0.2-μm polyvinylidene difluoride membrane (1620177; Bio-Rad, Hercules, CA) and were detected using an anti-HA antibody (1:1000; 3724; Cell Signaling Technologies), anti–T cell immunoglobulin cumin 1/kidney injury molecule-1/hepatitis A virus cellular receptor (TIM-1/KIM-1/HAVCR) antibody (1:1000; AF1817; Novus Biologicals, Littleton, CO), or anti–β-tubulin antibody (1:1000; 2146; Cell Signaling Technologies) as primary antibodies and rabbit anti–goat–horseradish peroxidase IgG (1:1000; HAF017; Novus Biologicals) or anti-rabbit IgG horseradish peroxidase–linked antibody (1:2000; 7074; Cell Signaling Technologies) as the secondary antibody. .. Blood urea nitrogen and serum creatinine were measured using the QuantiChrom Urea Assay Kit (DIUR-100; BioAssay Systems, Hayward, CA) and Creatinine Assay Kit (265-30; Sekisui Diagnostics P.E.I.

    Virus:

    Article Title: Matrix Gla Protein Expression in Pericytes and Myofibroblasts Contributes to Renal Fibrosis
    Article Snippet: .. Serum immunoprecipitants or kidney lysates were resolved on Tris-glycine SDS-PAGE gel, transferred onto a 0.2-μm polyvinylidene difluoride membrane (1620177; Bio-Rad, Hercules, CA) and were detected using an anti-HA antibody (1:1000; 3724; Cell Signaling Technologies), anti–T cell immunoglobulin cumin 1/kidney injury molecule-1/hepatitis A virus cellular receptor (TIM-1/KIM-1/HAVCR) antibody (1:1000; AF1817; Novus Biologicals, Littleton, CO), or anti–β-tubulin antibody (1:1000; 2146; Cell Signaling Technologies) as primary antibodies and rabbit anti–goat–horseradish peroxidase IgG (1:1000; HAF017; Novus Biologicals) or anti-rabbit IgG horseradish peroxidase–linked antibody (1:2000; 7074; Cell Signaling Technologies) as the secondary antibody. .. Blood urea nitrogen and serum creatinine were measured using the QuantiChrom Urea Assay Kit (DIUR-100; BioAssay Systems, Hayward, CA) and Creatinine Assay Kit (265-30; Sekisui Diagnostics P.E.I.



    Similar Products

    94
    Cytiva Europe pore size polyvinylidene difluoride membranes
    Pore Size Polyvinylidene Difluoride Membranes, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/Amersham+Protran+supported+Western+blot+membrane/pm42277016-423-13-18
    Average 94 stars, based on 1 article reviews
    pore size polyvinylidene difluoride membranes - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    86
    Amersham Life Sciences Inc polyvinylidene difluoride pvdf membrane
    Polyvinylidene Difluoride Pvdf Membrane, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/difluoride+membrane+polyvinylidene/pmc13196150-505-14-19
    Average 86 stars, based on 1 article reviews
    polyvinylidene difluoride pvdf membrane - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    Cytiva Europe polyvinylidene difluoride pvdf membranes
    Polyvinylidene Difluoride Pvdf Membranes, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/Amersham+Hybond+P+Western+blot+PVDF+membrane/pm42262889-804-15-20
    Average 95 stars, based on 1 article reviews
    polyvinylidene difluoride pvdf membranes - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Agrisera polyvinylidene difluoride membrane
    Polyvinylidene Difluoride Membrane, supplied by Agrisera, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/difluoride+membrane+polyvinylidene/pm42234757-371-10-21
    Average 86 stars, based on 1 article reviews
    polyvinylidene difluoride membrane - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    Bio-Rad polyvinylidene difluoride membranes
    Polyvinylidene Difluoride Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/Trans-Blot+Turbo+Mini+PVDF+Transfer+Packs/pmc13018931-112-23-27
    Average 96 stars, based on 1 article reviews
    polyvinylidene difluoride membranes - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Amersham Life Sciences Inc polyvinylidene difluoride pvdf membranes
    Polyvinylidene Difluoride Pvdf Membranes, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/membrane+pvdf+%EB%8B%A8%EB%B0%B1%EC%A7%88%EC%9D%84+%EC%98%81%EB%8F%99%EB%90%9C+%EC%A0%84%EA%B8%B0/pm42152682-83-15-19
    Average 86 stars, based on 1 article reviews
    polyvinylidene difluoride pvdf membranes - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    Bio-Rad polyvinylidene difluoride pvdf 1620177 membrane
    Polyvinylidene Difluoride Pvdf 1620177 Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/Immun-Blot+PVDF+Membrane/10__3390_slash_md24050179-149-13-20
    Average 96 stars, based on 1 article reviews
    polyvinylidene difluoride pvdf 1620177 membrane - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Epizyme Inc polyvinylidene difluoride pvdf membranes
    Polyvinylidene Difluoride Pvdf Membranes, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/membranes+pvdf/pm42139901-79-15-19
    Average 86 stars, based on 1 article reviews
    polyvinylidene difluoride pvdf membranes - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Burlington Industries polyvinylidene difluoride pvdf membrane
    Polyvinylidene Difluoride Pvdf Membrane, supplied by Burlington Industries, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyvinylidene+difluoride+membrane/membranes+pvdf/pm42139899-100-15-19
    Average 86 stars, based on 1 article reviews
    polyvinylidene difluoride pvdf membrane - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results